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  • For CRISPR, tweaking DNA fragments before inserting yields highest efficiency rates yet

    Professor Huimin Zhao led a team that achieved the highest reported efficiency of inserting genes into human cells with CRISPR-Cas9.

    Professor Huimin Zhao led a team that achieved the highest reported efficiency of inserting genes into human cells with CRISPR-Cas9.

    Photo by L. Brian Stauffer

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  • Editor’s notes: To reach Huimin Zhao, call (217) 333-2631; email: zhao5@illinois.edu.

    The paper “An efficient gene knock-in strategy using 5′-modified double-stranded DNA donors with short homology arms” is available online. DOI: 10.1038/s41589-019-0432-1